现代中药研究与实践

目的 优化桂龙巴布贴提取工艺参数。方法 以HPLC法测定方中桂皮醛(Cin)、香蒲新苷(Typ)、异鼠李素-3-O-新橙皮苷(Iso)含量及干膏率为评价指标，采用层次分析-熵权法(AHP-EWM)对权重系数进行计算，结合响应面法(Box-Behnken)优选桂龙巴布贴提取工艺。结果 确定Cin、Typ、Iso含量、干膏率的权重系数分别为0.844 5、0.091 5、0.056 4、0.007 6。经综合评分后，建立了预测数学模型。确定最佳工艺为优选的工艺为：加水回流提取2次，每次加10倍量水，提取70 min，滤过。在此条件下的综合评分为98.702，与模型预测值99.361差值较小，RSD小于3%，工艺稳定。结论 拟定的桂龙巴布贴提取工艺稳定可行，为临床更好地开发利用桂龙巴布贴提供了科学依据和技术支持。

Objective To optimize the extraction process parameters of Guilong cataplasm.Methods The HPLC method was used to determine the components of cinnamaldehyde,chamomile neoside,isorhamnetin-3-O-neohesperidin and the dry extract rate of the formula as the evaluation indexes,and the weight coefficients were calculated by using AHP-EWM,and the extraction process of Guilong cataplasm was preferred by combining the Box-Behnken Response Surface Method.Results The weighting coefficients of Cin,Typ,Iso content and dry paste rate were determined as 0.844 5,0.091 5,0.056 4 and 0.007 6,respectively.After the combined scoring,the predictive mathematical model was developed.The optimal preparation was as follows:heated and refluxed for twice,added 10 times the amount of water and 70 min for each time.The composite score under this condition was 98.702,which was a very small difference from the model prediction of 99.361,with an RSD of less than 3%,and the process was stable.Conclusion The proposed extraction process of Guilong cataplasm is stable and feasible,which provides scientific basis and technical support for better clinical development and utilisation of Guilong cataplasm.