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目的 探究甘草地上部分提取物(GU)对于角叉菜胶诱导的大鼠慢性前列腺炎/慢性盆腔疼痛综合征(CP/CPPS)的作用机制。方法 采用乙醇提取后水沉的方法制备GU，测定其总黄酮含量确定给药量。建立角叉菜胶诱导的CP/CPPS大鼠模型，将大鼠分为对照组、假手术组、模型组和给药组，连续灌胃给药14 d，测量GU对CP/CPPS大鼠的前列腺系数、血清中IL-6和TNF-α的水平，苏木精-伊红(HE)染色观察大鼠前列腺组织内病理学变化。结合转录组学和代谢组学对大鼠前列腺组织进行测序和筛选差异代谢物，进行相关性分析获得关键基因。结果 血清生化结果表明，GU降低了CP/CPPS大鼠血清内IL-6和TNF-α的水平，降低了炎症反应。HE结果表明GU显著减少了CP/CPPS大鼠前列腺组织内炎症细胞的浸润。代谢组学和转录组学联合分析筛选出SOCS3、FXYD5、AIM2、PLA2G2A、CXCL1和HSD17B6共6个关键基因。差异基因的KEGG富集于病毒蛋白与细胞因子和细胞因子受体的相互作用、NF-κB 等信号通路，差异代谢物的KEGG富集与精氨酸生物合成，丙氨酸、天门冬氨酸和谷氨酸代谢等代谢过程密切相关。结论 GU可能通过抑制角叉菜胶诱导的CP/CPPS大鼠的炎症反应、细胞增殖等过程发挥抗CP/CPPS作用。
Abstract：Objective To investigate the mechanism of action of the aboveground part of Glycyrrhiza uralersis Fisch.extract (GU) on carrageenan gum-induced chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) in rats.Methods GU was prepared by ethanol extraction followed by water precipitation,and its total flavonoid content was determined to determine the dosage.A rat model of CP/CPPS induced by carrageenan gum was established,and the rats were divided into the control group,the sham operation group,the model group and the administration group for 14 d by continuous gavage.The prostate coefficients,serum levels of IL-6 and TNF-α in rats with CP/CPPS were measured by GU,and the pathological changes in the prostate tissue of rats were observed by hematoxylin-eosin (HE) staining.Combined with transcriptomics and metabolomics,rat prostate tissues were sequenced and screened for differential metabolites,and correlation analysis was performed to obtain the key genes and metabolites.Results Serum biochemical results showed that GU reduced the levels of IL-6 and TNF-α within the serum of CP/CPPS rats,indicating reduced inflammatory response.HE results showed that GU significantly reduced the infiltration of inflammatory cells within the prostate tissue of CP/CPPS rats.Combined metabolomics and transcriptomics analysis screened a total of 6 key genes and 17 key metabolites,including SOCS3,FXYD5,AIM2,PLA2G2A,CXCL1 and HSD17B6.KEGG enrichment of differential expressed genes was found in signaling pathways such as interactions of viral proteins with cytokines and cytokine receptors,NF-κB,etc.KEGG enrichment of differential expressed metabolites was closely related to metabolic processes such as arginine biosynthesis,and metabolism of alanine,aspartate and glutamate.Conclusion GU can exert its anti-CP/CPPS effects by inhibiting processes such as inflammatory response and cell proliferation in CP/CPPS rats.
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基本信息:
DOI：10.13728/j.1673-6427.2025.04.004
中图分类号:R285.5
引用信息:
[1]云鹭,张艳,施露等.基于转录组学与代谢组学对甘草地上部分提取物改善慢性前列腺炎作用机制研究[J].现代中药研究与实践,2025,39(04):20-26.DOI:10.13728/j.1673-6427.2025.04.004.
基金信息:
国家重点研发计划项目(2018YFC1706500); 国家工信部中药材提升和保障项目(2020071620161); 北京中医药大学22年度纵向科研发展基金(90020272120022)