现代中药研究与实践

目的 研究四氮唑染色法(TTC法)测定百蕊草含胚种子生活力的最佳条件，建立百蕊草种子生活力的快速检验方法。方法 以自然干燥、阴凉处储存的饱满百蕊草种子为材料，采用TTC染色法，研究不同预措方法对百蕊草含胚种子生活力的影响，通过单因素试验、正交试验研究不同预湿温度、预湿时间、染色时间、染色温度、TTC浓度对百蕊草含胚种子生活力的影响。结果 纵剖处理是最佳预措方式；5个因素对百蕊草含胚种子生活力的影响顺序依次为染色时间>染色温度>TTC浓度>预湿温度>预湿时间；正交试验染色最佳条件是种子于30 ℃预湿6 h，以0.3%TTC在35 ℃染色12 h。结论 优化后的TTC染色法可快速测定百蕊草含胚种子生活力。

Objective To explore the optimal conditions of tetrazolium staining method (TTC method) for determining the viability of embryo-containing seeds of Thesium chinense Turcz.,and to establish a rapid test method for the viability of seeds of Thesium chinense Turcz..Methods Naturally dried and stored in a cool place of full Thesium chinense Turcz.seeds were used as materials.TTC staining was used to study the effects of different pre-conditioning methods on the viability of embryo-containing seeds of Thesium chinense Turcz..The effects of different pre-wetting temperatures,pre-wetting times,staining times,staining temperatures,and TTC concentration on the viability of embryo-containing seeds of Thesium chinense Turcz.were investigated through one-way and orthogonal tests.Results The longitudinal treatment was the best pre-treatment method;the effects of the five factors on the embryonic seed viability of Thesium chinense Turcz.were in the order of staining time>staining temperature>TTC concentration>pre-wetting temperature>pre-wetting time;the best condition for staining in orthogonal test was that the seeds were pre-wetted at 30 ℃ for 6 h,and then stained with 0.3% TTC at 35 ℃ for 12 h.Conclusion The optimised TTC staining method is able to quickly determine the viability of embryo-containing seeds of Thesium chinense Turcz..